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so I have no conflict of interest to declare so the goal of my presentation is to introduce different stimulation chemo genetics and also their applications in the preclinical fMRI field I also like to discuss the pros and cons as well as to do's and don'ts with these techniques and also lastly I'll share my own experience regarding how to get these experiments starting your own laboratory so electrical deep brain stimulation or DBS is a very well-established in your surgical therapy for many neurological and psychiatric disorders in human patients electro is typically implanted at the different nucleus and then high-frequency stimulation is usually given through subcutaneous like pacemakers the immersion device so it is most commonly used to treat disease such as Parkinson's disease usually when the medical therapies becomes inadequate or dyskinesia becomes intolerable and when we use