*Note: -Enzymatic process breaks the phosphatides into two parts. One part will be a lipophilic byproduct which requires additional winterization to fully remove. The second is hydrophilic which willrequire a heptane/water wash to remove. It is recommended to perform the degumming process priorto winterization for best results.

Do not subject D-Gum powder to temperatures >50°C (Ideal temperature for enzymatic activity is 40°C) A

Do not expose D-Gum to Alcohol or other solvents during incubation as this may de-nature the enzymes and render them useless.

Required Materials:

Heptane

Water

Heat plate/Mag Stirrer

pH Meter/Test Strips

Thermometer

Homogenizer

High-accuracy scale

Rotovap

Sep Funnel/Reactor