Persimmon Endosperm, l.s. Microscope Slide

prepare the filtration system turn on the vacuum pump and rinse the filtration system with sterile water then place the backing filter on the filter base it doesn't matter which side is up and wet the filter with sterile water turn on the pump and then place the membrane filter on top of the wet backing filter with the shiny side up and avoid air bubbles and wrinkles you can turn the vacuum pump on again now clamp on the filter funnel and gently homogenize the water sample and pipette in appropriate volume of sample into the funnel and turn the vacuum pump on and filter to dry so until all the sample is filtered add a few drops of acridine orange solution using the syringe and the syringe filter and cover the funnel with aluminum foil and let the equity in orange stay in the cells for three minutes in the meantime add a drop of immersion oil to the center of the slide and spread it then